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Acsl4 fl/fl mice were obtained from Cyagen Biosciences (Jiangsu), and Fsp1 fl/fl mice and Ksp Cre-ERT2 mice were sourced from GemPharmatech (Jiangsu)

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The second is the "sealed defense" of tight junctions, which maintains the low permeability of the barrier and prevents the leakage of harmful substances by regulating the expression and distribution of tight junction proteins

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