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Insulin-like Growth Factor-1 Long R3 is a modified IGF-1 with an extended half-life

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Splenocytes were washed with warm RPMI 1640, stained at 37 C for 30 min with Abs diluted in RPMI 1640, and washed with PBS

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Endogenous peroxidase was blocked in 5% H 2 O 2 (3 10 min), and then, sections were incubated with primary mouse anticytokeratin 18, clone DC10 (DAKO, Glostrup, Denmark