The needle should slide in easily with minimal resistance
Lipsitch M, Tchetgen Tchetgen E, Cohen T (2010) Negative controls: a tool for detecting confounding and bias in observational studies
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When injections are irregular, people report creeping hunger at different times, protein goals slip, and mild nausea becomes a reason to skip doses rather than a manageable side effect
Furthermore, stimulation of the vagal nerve in mice after administering lipopolysaccharides (LPS) suppresses the inflammation-induced production of TNF- and interleukin (IL)-6 by microglia, which is not observed in vagotomized mice 20
10.1007/BF02190088 69