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Images were collected with a 40 lens (Zeiss Objective C-Apochromat 40/1.2 W Corr M27) in multi-track mode with line switching between 488 nm excitation and 405 nm excitation and taking an average of four readings in case of GRX1-roGFP2 and two readings for GRX1-roGFP2-iL, respectively

It is unclear whether loss of GPx-1 is causative or whether it occurs secondary to cellular transformation to promote subsequent tumor growth (presumably by enhanced hydrogen peroxide-mediated growth factor responses)