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Do not panic, and do not suspend treatment

When confluent, HAECs were shifted to ALI culture with 50 L apical media for 8 days to achieve full differentiation with and without IL-13 (10 ng/mL) stimulation (R&D Systems, 213-ILB)

Include cooked vegetables, berries, prunes, and chia seeds, which retain moisture in stool and promote movement

Adjust draw volume accordingly, or discard and start over with a new vial if the math is hard to reconcile

It is intended as a raw material for scientific research and experimental development

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