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After centrifugation, the pellet was resuspended in red cell lysis buffer (150 mM NaH 4 Cl, 10 mM NaHCO 3 , 1.27 mM EDTA) and incubated for 2 min at RT with gentle manual agitation and centrifuged again

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Effects of metformin on body weight and body composition in obese insulin-resistant children: a randomized clinical trial

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