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Purified MDH (~1 mL) was dialyzed for 4 h in 1 L buffer 2 (20 mM MOPS-KOH pH 7.5, 150 mM NaCl) containing 10 mM EDTA followed by three times dialysis against 1 L of buffer 2

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National Health and Nutrition Examination Survey (NHANES), which included 2,154 adults aged 60 years and older, found that vitamin D, as a mediator related to oxidative stress, significantly mediated the relationship between Se intake and cognitive function, accounting for 8.02% of the association