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Therefore, the amphipathic helices of Cr FAP65 (WT and the variants) were individually gene synthesized in pGEX-4T-1 and the choice of the host was E

Proteins were separated by sodium dodecyl sulfate polyacrylamide gel electrophoresis and transferred to polyvinylidene difluoride membranes (Millipore, Bedford, MA, USA)

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