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(2) molecular dynamics simulated annealing restraining FOXO4 FH (without flexible regions) harmonically to its template structure (using a non-crystallographic force constant of 10,000 kcal mol 1 2 ), with additional dihedral angle restraints from 1 H, 15 N, and 13 C chemical shifts using TALOS-N 56 , hydrogen bond restraints (region p53 residues 48-55

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Bands were developed using Clarity Western ECL substrate (Bio-Rad Laboratories, Hercules, CA, USA)

Beyond its primary roles in hydrochloric acid secretion and pepsinogen-mediated protein digestion, this niche also functions as a biological filter that restricts microbial passage

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