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This makes the histidine-Cu 2+ interactions and subsequent conformational changes of PrPc upon Cu 2+ binding pH dependent ( 2+ binding (anchoring) is followed when Cu 2+ is in a coordinated state, and this is achieved by the presence of two deprotonated backbone amides ( 2+ involves less than 6 ions

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By the end, air exchange through those accumulated micro-channels is significant

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Required storage: Cool, dry place protected from light