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Dissolution in suitable solvent (e.g., water + acetonitrile) Reduction and alkylation (if disulfide bonds are present) Enzymatic digestion (optional for bottom-up sequencing approach) Key Insight: Clean and well-prepared samples significantly reduce noise and improve detection sensitivity

Effect of cooking on total vitamin C contents and antioxidant activity of sweet chestnuts

doi: 10.1016/j.abb.2019.07.008

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coli expressing glutamate dehydrogenase (PpGluDH) and glucose dehydrogenase (BsGDH-2M, SEQ ID NO