After 21 days of osteogenic differentiation with or without exposure to nicotine or CSE, the alkaline phosphatase (ALP) activity of MSCs was measured using 4-nitrophenyl phosphate as described previously 19
Studies were identified by searching relevant papers via PubMed/MEDLINE ( Cochrane Library and EMBASE using the following keywords: (antidepressive agents) AND (smok*)
However, we addressed these concerns by testing alternative change years from 2008 to 2018
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